Zhang, Y., Malzahn, A. A., Sretenovic, S. & Qi, Y. The emerging and uncultivated potential of CRISPR technology in plant science. Nat. Plants 5, 778–794 (2019).

The exploitation of CO2 capture, utilisation and storage technologies (CCUS) in industrial applications face significant challenges due to the high investment cost and the fierce international competition in the sectors concerned. Nonetheless, it is widely known that these industrial sectors currently account for 20% of global CO2 emissions, and according to the 2-degree scenario of the Paris agreement, they should represent half of the stored CO2 by 2050. In this frame, relevant sectors with high CO2 emissions are for example steel, iron and cement making, biofuel production and waste incineration plants, oil refining, gas processing, hydrogen production. The CooCE project has an absolute aim to accelerate the use of CCUS and revolutionize CO2 capture and utilization by closing carbon loops in a circular economy approach. Nishimasu, H. et al. Engineered CRISPR–Cas9 nuclease with expanded targeting space. Science 361, 1259–1262 (2018). Governance: This Project is an ‘Accelerating CS Technologies’ (ACT) funding initiative which involves the following Multi-National Collaborators; TWI Ltd (Project Co-Ordinator), University of Leicester, Technovative Solutions Ltd, Pilkington Technical Management Ltd, ETHNIKO KENTRO EREVNAS KAI TECHNOLOGIKIS ANAPTYXIS (Centre for Research & Technology Hellas - CERTH) and Battelle Energy Alliance, LLC One day Peter and John ( A) were going up to the temple ( B) at the time of prayer—at three in the afternoon. ( C) 2 Now a man who was lame from birth ( D) was being carried to the temple gate ( E) called Beautiful, where he was put every day to beg ( F) from those going into the temple courts. 3 When he saw Peter and John about to enter, he asked them for money. 4 Peter looked straight at him, as did John. Then Peter said, “Look at us!” 5 So the man gave them his attention, expecting to get something from them. Investigate the potential for CO2 delivery from CLC WtE plants for permanent storage at Northern Lights (Longship) and/or CO2 utilisation

4 Core Cables

Zhang, Y. et al. Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells. Sci. Rep. 4, 5405 (2014).

This project aim to accelerate a new carbon capture and utilization technology for large CO2 emitters through the conversion of waste heat and industrial CO2 streams into valuable products. The core new technology is based on the Carbonova process that is a unique revolutionary chemical process with novel catalysts and equipment. This process uses carbon dioxide and natural gas in a chemical reactor, where they are combined with waste heat from other processes (such as a cement production or a power plant)) and converted into the carbon nanofibres (CNF). WP5 - Enabling 'cold CO2 injection' into depleted reservoirs. This WP fulfils two main objectives: (i) Coordination of WPs 2-4 to ensure a targeted working process and progress and (ii) The delivery of a compilation of practical recommendations for safe and cost efficient CO2 injections into depleted reservoirs. An essential part of this activity are yearly industry workshops. The workshops will provide direction and input to the technical work in WP2-4 and enable the exchange of expectations, knowledge and data between the operators and researchers for a proper design of case studies and application scenarios.WP2 will expose sealants to supercritical CO2 containing H2S as well as other common impurities, to investigate changes in mineral composition. Khanday, I., Skinner, D., Yang, B., Mercier, R. & Sundaresan, V. A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds. Nature 565, 91–95 (2019). Lowder, L. G. et al. Robust transcriptional activation in plants using multiplexed CRISPR–Act2.0 and mTALE–Act systems. Mol. Plant 11, 245–256 (2018). Qin, P. et al. Live cell imaging of low- and non-repetitive chromosome loci using CRISPR–Cas9. Nat. Commun. 8, 14725 (2017).

Xie, K., Minkenberg, B. & Yang, Y. Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc. Natl Acad. Sci. USA 112, 3570–3575 (2015).

Play Act 2 Scene 2

Act 3, scene 4 As Macbeth’s banquet begins, one of Banquo’s murderers appears at the door to tell Macbeth of Banquo’s death and Fleance’s escape. Returning to the table, Macbeth is confronted by Banquo’s ghost, invisible to all but Macbeth. While Lady Macbeth is able to dismiss as a momentary fit Macbeth’s expressions of horror at the ghost’s first appearance, the reappearance of the ghost and Macbeth’s outcries in response to it force Lady Macbeth to send all the guests away. Alone with Lady Macbeth, Macbeth resolves to meet the witches again. He foresees a future marked by further violence. WP6 - Outreach and dissemination. WP6 aims to ensure an effective communication within the consortium, including associated partners, and to disseminate project information and results. The WP will ensure that relevant findings are shared within and outside the CCUS community using modern and accessible methods. Prospero then uses his magic to put Miranda to sleep and calls to his spirit servant Ariel. Ariel describes how he created the storm and that the ship is now ‘Safely in harbour’ and everyone on board is safe. Prospero is pleased but tells Ariel ‘there’s more work’. Ariel objects and Prospero threatens him but he also promises the spirit his freedom ‘after two days’ if he obeys. The theme of love is always important in Shakespeare’s comedies. Ferdinand and Caliban are the only two men Miranda has known, apart from her father. As potential lovers to Miranda, compare how Ferdinand and Caliban are described and consider why Prospero wants his daughter to marry one and not the other. CoCaCO2la will use Cu as electrocatalyst by coating it onto the electrode surface via industrial methods such as thermal/cold spray and benchmark it against electrodeposition. These allow cost-effective production route (with a potential for industrial uptake) in addition to the versatility of ease of repair and re-use. The microstructure of the coated Cu layer can be modified by changing the process parameters, enabling high ethylene selectivity.

Konermann, S. et al. Genome-scale transcriptional activation by an engineered CRISPR–Cas9 complex. Nature 517, 583–588 (2015). Li, Z. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nat. Plants 3, 930–936 (2017). Xu, Z. et al. SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition. Genome Biol. 22, 6 (2021). Corded sensor probe and extendable tubing enable the humidifier to be positioned outside the grow tent.

Play Act 1 Scene 2

Act 1, scene 4 Duncan demands and receives assurances that the former thane of Cawdor has been executed. When Macbeth, Banquo, Ross, and Angus join Duncan, he offers thanks to Macbeth and Banquo. He then announces his intention to have his son Malcolm succeed him as king and his plan to visit Macbeth at Inverness. Macbeth sets out ahead of him to prepare for the royal visit. Now that Malcolm has been named Duncan’s successor, Macbeth is convinced that he can become king only by killing Duncan. Alone on stage, Lady Macbeth expresses her unhappiness: there seems to be no end to her desire for power and she feels insecure and anxious. Macbeth enters looking upset and she counsels him to stop mulling over the crimes they have committed. But Macbeth declares that their job is not done: he still spends every waking moment in fear and every night embroiled in nightmares. He even envies Duncan, who now sleeps peacefully in his grave. Lady Macbeth warns him to act cheerful in front of their dinner guests. She also tries to comfort him by reminding him that Banquo and Fleance are by no means immortal. Macbeth responds by telling her that "a deed of dreadful note" will be done in the night, though he will not divulge the details (33). Act 3, Scene 3 Ma, H. et al. CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nat. Methods 15, 928–931 (2018). Zhang, Y. et al. A gRNA–tRNA array for CRISPR–Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae. Nat. Commun. 10, 1053 (2019). Meaco is a UK company that produces a wide range of air cooling, purification and dehumidifying products so it knows a thing or two about moving air and doing things to that air in the process. This new 14,000 BTU model is ideal for rooms between 25 and 35m² and features three fan speeds and a low temperature setting of 16ºC, which is the norm for the majority of portable air con units and indeed most cars.